16S rRNA Amplicon Sequencing

The service includes:

• QC of the DNA
• library preparation
• Sequencing on MiSeq 2x300 bp
• A turnaround time of approximately 3-4 weeks after submission
• Minimum 80,000 reads per sample

Region	Fragment length	Primer	Primer sequences (5’-3’)
V4	292 bp	515F	GTGCCAGCMGCCGCGGTAA
		806R	GGACTACHVGGGTWTCTAAT
V3-V4	466 bp	341F	CCTAYGGGRBGCASCAG
		806R	GGACTACNNGGGTATCTAAT

Number of samples	Price for UTS researchers	Price for external researchers
*1-48	$85	$95
More than 48	$75	$85

• There is a minimum number of samples submission needed to be eligible for this price.Please contact the facility for more details. For other amplicon sequencing services such as ITS, please contact the facility for a quote: ngsfacility@uts.edu.au